| Peer-Reviewed

Surveillance of Resistance to Imipenem and Meropenem in Broad-spectrum Beta-lactamase-producing E. coli Strains Isolated from Urine Samples in Njombe, Cameroon

Received: 31 December 2021     Accepted: 23 February 2022     Published: 23 March 2022
Views:       Downloads:
Abstract

The emergence and spread of carbapenems resistance Enterobacteriaceae remain a major public health, a real threat as well as a silent tsunami. This phenomenon leading to reduce the therapeutic option and increase the additional cost. The general objective of this study was to determine the frequency of Escherichia coli strains producing broad-spectrum beta lactamases and resistant to carbapenems. We carried out a descriptive, cross sectional and prospective study between August and November 2020 at the Hospital Saint Jean de Malte in Njombe, on a consecutive sample of 249 patients received at the bacteriology unit. Our study population consisted of all patients who came for inpatient or outpatient consultations, were prescribed an cytobacterioligical urine exam, and in whom an E. coli strain was isolated. The identification of E. coli strains was confirmed using the Api 20E mini gallery. Resistance to carbapenems (Meropenem and Imipenem) was defined by determining the Minimal Inhibitory Concentration (MIC) by the microdilution plate method. Of the 249 cytobacterioligical urine exam samples received during our study period, 131 presented a pathogenic germ and E. coli strains were identified in 85 of them. The age of the patients in whom the E. coli strains were identified ranged from 70 to 85 years and the male sex dominated with a frequency of 38.2%. Of these identified E. coli strains, we detected 08 (9.41%) BLSE-producing strains. The resistance rate of the isolated E. coli strains producing BLSE was 75% and 50% for Meropenem and Imipenem respectively. The results of this study underline the urgent need for a regular surveillance system for broad-spectrum antibiotics in our context.

Published in International Journal of Microbiology and Biotechnology (Volume 7, Issue 1)
DOI 10.11648/j.ijmb.20220701.15
Page(s) 37-42
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Resistance, Imipenem, Meropenem, E. coli, Broad-spectrum Beta-lactamases

References
[1] Pitout, Johann D. D., Patrice Nordmann, Kevin B. Laupland, et Laurent Poirel. Emergence of Enterobacteriaceae Producing Extended-Spectrum Beta-Lactamases (ESBLs) in the Community. The Journal of Antimicrobial Chemotherapy. Vol. 56, No. 1, 2005, pp. 52-59. https://doi.org/10.1093/jac/dki166.
[2] Rakotovao-Ravahatra, Zafindrasoa Domoina, Fidiniaina Mamy Randriatsarafara, Saïda Rasoanandrasana, Léa Raverohanta, et Andriamiadana Luc Rakotovao. Phénotypes de résistance des souches d’Escherichia coli responsables d’infection urinaire au laboratoire du Centre Hospitalo-Universitaire de Befelatanana Antananarivo [Resistant phenotypes of Escherichia coli strains responsible for urinary tract infection in the laboratory of the University Hospital Joseph Raseta Befelatanana, Antananarivo]. The Pan African Medical Journal. Vol. 26, No. 166, 2017. https://doi.org/10.11604/pamj.2017.26.166.11828.
[3] Aenishaenslin, Cécile, Barbara Häsler, André Ravel, Jane Parmley, Katharina Stärk, et David Buckeridge. Evidence Needed for Antimicrobial Resistance Surveillance Systems. Bulletin of the World Health Organization. Vol. 97, No 4 2019, pp. 283-89. https://doi.org/10.2471/BLT.18.218917.
[4] Madhi, Fouad, Camille Jung, Sandra Timsit, Corinne Levy, Sandra Biscardi, Mathie Lorrot, Emmanuel Grimprel, et al. Febrile urinary-tract infection due to extended-spectrum beta-lactamase–producing Enterobacteriaceae in children: A French prospective multicenter study. PLoS ONE. Vol. 13, No. 1, 2018https://doi.org/10.1371/journal.pone.0190910.
[5] Sbiti, Mohammed, Khalid Lahmadi, et Lhoussaine Louzi. Profil Épidémiologique Des Entérobactéries Uropathogènes Productrices de Bêta-Lactamases à Spectre Élargi [Epidemiological profile of uropathogenic enterobacteria producing extended spectrum beta-lactamases]. The Pan African Medical Journal. Vol. 28, No. 29, 2017. https://doi.org/10.11604/pamj.2017.28.29.11402.
[6] Souha, S, MD Kanj, A Zeina, et MD Kanafani. Current concepts in antimicrobial therapy against resistant gram-negative organisms: extended-spectrum beta-lactamase-producing Enterobacteriaceae, carbapenem-resistant Enterobacteriaceae, and multidrug-resistant Pseudomonas aeruginosa. Mayo Clinic proceedings. Vol. 86, No. 3, 2011, pp. 250–259. doi: 10.4065/mcp.2010.0674.
[7] Arpin, Corinne, Véronique Dubois, Laure Coulange, Catherine André, Isabelle Fischer, Patrick Noury, Frédéric Grobost, et al. Extended-Spectrum β-Lactamase-Producing Enterobacteriaceae in Community and Private Health Care Centers. Antimicrobial Agents and Chemotherapy. Vol. 47, No. 11, 2003, pp. 3506-14. https://doi.org/10.1128/AAC.47.11.3506-3514.2003.
[8] Rodríguez-Baño, Jesús, Belén Gutiérrez-Gutiérrez, Isabel Machuca, et Alvaro Pascual. Treatment of Infections Caused by Extended-Spectrum-Beta-Lactamase-, AmpC-, and Carbapenemase-Producing Enterobacteriaceae. Clinical Microbiology Reviews. Vol. 31, No. 2, 2018, pp. 79-17. https://doi.org/10.1128/CMR.00079-17.
[9] Harris, Patrick N. A., Paul A. Tambyah, David C. Lye, Yin Mo, Tau H. Lee, Mesut Yilmaz, Thamer H. Alenazi, et al. Effect of Piperacillin-Tazobactam vs Meropenem on 30-Day Mortality for Patients With E Coli or Klebsiella Pneumoniae Bloodstream Infection and Ceftriaxone Resistance: A Randomized Clinical Trial. JAMA. Vol. 320, No. 10, 2018, pp. 984-994. doi: 10.1001/jama.2018.12163.
[10] Ouedraogo, Abdoul-Salam. Prévalence, circulation et caractérisation des bactéries multirésistantes au Burkina Faso [Prevalence, circulation and characterization of multi-drug resistant bacteria multidrug-resistant bacteria in Burkina Faso]. Phd thesis, Université Montpellier, 2016. https://tel.archives-ouvertes.fr/tel-01476152.
[11] Codjoe, Francis S., et Eric S. Donkor. Carbapenem Resistance: A Review. Medical Sciences. Vol. 6, No. 1, 2017, pp. 1. https://doi.org/10.3390/medsci6010001.
[12] Meletis, Georgios. Carbapenem resistance: overview of the problem and future perspectives. Therapeutic Advances in Infectious Disease. Vol. 3, No. 1, 2016, pp. 15-21. https://doi.org/10.1177/2049936115621709.
[13] Mouiche, Mohamed Moctar Mouliom, Frédéric Moffo, Jane-Francis Tatah Kihla Akoachere, Ndode Herman Okah-Nnane, Nabilah Pemi Mapiefou, Valantine Ngum Ndze, Abel Wade, et al. Antimicrobial Resistance from a One Health Perspective in Cameroon: A Systematic Review and Meta-Analysis. BMC Public Health. Vol. 19, No. 1135, 2019. https://doi.org/10.1186/s12889-019-7450-5.
[14] Mitgang, Elizabeth A., David M. Hartley, Marissa D. Malchione, Michala Koch, et Jesse L. Goodman. Review and Mapping of Carbapenem-Resistant Enterobacteriaceae in Africa: Using Diverse Data to Inform Surveillance Gaps. International Journal of Antimicrobial Agents. Vol. 52, No. 3, 2018, pp. 372-84. doi: 10.1016/j.ijantimicag.2018.05.019.
[15] Franqueville André, Champaud Jacques (collab.), Elingui H. (collab.). Dictionnaire des villages du Mungo [Dictionary of the villages of Mungo]. Yaoundé ORSTOM, 1971, X-33 p. NOBRA SH 62.
[16] Bush, Karen, et Patricia A. Bradford. Epidemiology of β-Lactamase-Producing Pathogens. Clinical Microbiology Reviews. Vol. 33, No. 2, 2020, pp. 47-19. https://doi.org/10.1128/CMR.00047-19.
[17] Nascimento, Júllia A. S., Fernanda F. Santos, Tiago B. Valiatti, José F. Santos-Neto, Ana Carolina M. Santos, Rodrigo Cayô, Ana C. Gales, et Tânia A. T. Gomes. Frequency and Diversity of Hybrid Escherichia coli Strains Isolated from Urinary Tract Infections. Microorganisms. Vol. 9, No. 4, 2021, pp. 693. https://doi.org/10.3390/microorganisms9040693.
[18] Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing, 30th ed. Clinical & Laboratory Standards Institute, 2020. https://clsi.org/standards/products/microbiology/documents/m100/.
[19] Bao, Lidao, Rui Peng, Xianhua Ren, Ruilian Ma, Junping Li, et Yi Wang. Analysis of Some Common Pathogens and Their Drug Resistance to Antibiotics. Pakistan Journal of Medical Sciences. Vol. 29, No. 1, 2013, pp. 135-39. https://doi.org/10.12669/pjms.291.2744.
[20] Rangaiahagari, A., J. P. Uwizeyimana, J. Nyirabanzi, E. Ngoga, et J. Wane. Antibiotic Sensitivity Patterns of Enterobacteriaceae Isolated At King Faisal Hospital, Kigali - A Three Years Study. Antibiotic sensitivity patterns. Vol. 70 No. 1, 2013, pp. 11–14. https://tspace.library.utoronto.ca/handle/1807/41177.
[21] Garba, Abdoul-Aziz, Mahamadou Doutchi, Maman Lawali, Hassan Diongole, Maazou Halidou, Iliassou Aboubacar, Ibrahim Alkassoum, et Eric Adehossi. Étude Bactériologique Des Infections Urinaires Chez l’Adulte Au Laboratoire de Microbiologie de l’Hôpital National de Zinder [Bacteriological profile and antibiotic sensitivity of urine culture isolates in the Microbiology Laboratory of Zinder National Hospital]. Health sciences and disease. Vol. 21, No. 3, 2020. https://www.hsd-fmsb.org/index.php/hsd/article/view/1872.
[22] Bertholom, Chantal. Épidémiologie des infections urinaires communautaires et nosocomiales [Epidemiology of community and nosocomial urinary tract infections]. Option/Bio. Vol. 27, No. 541, 2016, pp. 23–24. doi: 10.1016/s0992-5945(16)30116-7.
[23] Doublet, J.-D. À propos des recommandations actualisées pour le diagnostic et l’antibiothérapie des infections urinaires bactériennes de l’adulte [About the updated recommendations for the diagnosis and antibiotic therapy of bacterial urinary tract infections in adults]. Progrès En Urologie – FMC. Vol. 25, No. 4, 2015, pp. 99–105. http://dx.doi.org/ 10.1016/j.fpurol.2015.06.005.
[24] Wazieres, B de. Infections urinaires nosocomiales: qui traiter, quand traiter et comment traiter en gériatrie? [Nosocomial urinary tract infections: who, when, and how to treat in geriatric institutions?] Médecine et Maladies Infectieuses. Vol. 33, No. 9, 2003, pp. 469–473. https://doi.org/10.1016/S0399-077X(03)00149-5.
[25] Société de Pathologie Infectieuse de Langue Française (SPILF). Diagnostic et antibiotherapie des infections urinaires bactériennes communautaires de l’adulte [Diagnosis and antibiotic therapy of community-acquired bacterial urinary tract infections in adults], 2015.
[26] Joly Guillou, Marie Laure. Risque bactérien et personnes âgées [Bacterial risk and ederly population]. Revue Francophone des Laboratoires. Vol. 2016, No. 485, 2016, pp. 65–72. https://doi.org/10.1016/S1773-035X(16)30282-9.
[27] kalambry, Aime, N. Gaudré, Boubacar SI Drame, A. Poudiougo, A. Kassogué, H. Koné, et A. Diarra. Profil de résistance aux bêta-lactamines des entérobactéries isolées des prélèvements urinaires à l’Hôpital du Mali [Antibiotic resistance profile of enterobacteria isolated from urinary samples at Hospital Mali]. Revue Malienne d’Infectiologie et de Microbiologie. Vol. 14, No. 2, 2019, pp. 6–13. https://doi.org/10.53597/remim.v14i2.1363.
[28] Nicolas-Chanoine, Marie-Hélène, Coraline Gruson, Suzanne Bialek-Davenet, Xavier Bertrand, Frédérique Thomas-Jean, Frédéric Bert, Mati Moyat, et al. 10-Fold Increase (2006-11) in the Rate of Healthy Subjects with Extended-Spectrum β-Lactamase-Producing Escherichia Coli Faecal Carriage in a Parisian Check-up Centre. The Journal of Antimicrobial Chemotherapy. Vol. 68, No. 3, 2013, pp. 562-68. https://doi.org/10.1093/jac/dks429.
[29] Djim-Adjim-Ngana, Karyom, Leila Aïcha Oumar, Brunel Wanda Mbiakop, Hermann Landry Munshili Njifon, Tania Crucitti, Elias Nukenine Nchiwan, Nicolas Njintang Yanou, et Louis Deweerdt. Prevalence of extended-spectrum beta-lactamase-producing enterobacterial urinary infections and associated risk factors in small children of Garoua, Northern Cameroon. Pan African Medical Journal. Vol. 36, 2020, pp. 157. https://doi.org/10.11604/pamj.2020.36.157.21347.
[30] kone, J., B. Bellahcen, A. Awab, R. El Moussaoui, M. Alilou, A. El Hijri, et A. Azzouzi. Les entérobactéries sécrétrices de béta-Lactamases à spectre étendu en urologie à l’Hôpital Ibn Sina de Rabat [Extended spectrum beta-lactamase producing enterobacteriaceae in urology at Hospital Ibn Sina of Rabat]. Revue Malienne d’Infectiologie et de Microbiologie. Vol. 5, 2016. https://doi.org/10.53597/remim.v0i0.890.
[31] Okalla Ebongue, Cecile, M Tsiazok, JP Mefo’o, Guy Ngaba, G Beyiha, et D Adiogo. Evolution de la résistance aux antibiotiques des entérobactéries isolées à l’Hôpital Général de Douala de 2005 à 2012 [Evolution of antibiotic resistance in Enterobacteria isolated at the Douala General Hospital from 2005 to 2012]. Revue de Médecine et de Pharmacie. Vol. 4, 2014, pp. 393-99. doi: 10.11604/pamj.2015.20.227.4770.
[32] Lafaurie, M., et Raphaël Lepeule. Durée de Traitement Des Infections Urinaires [Duration of treatment of urinary tract infections]. Journal des Anti-infectieux. Vol. 15, 2013, pp. 119–132. https://doi.org/10.1016/j.antinf.2013.08.001.
[33] Sekhsokh, Y., M. Chadli, et S. A. El Hamzaoui. Fréquence et sensibilité aux antibiotiques des bactéries isolées dans les urines [Frequency and antibiotic susceptibility of bacteria identified in urine]. Médecine et Maladies Infectieuses. Vol. 38, No. 6, 2008, pp. 324–327. https://doi.org/10.1016/j.medmal.2008.02.003.
Cite This Article
  • APA Style

    Marthe Viviane Fonkam, Cédric Gueguim, Michel Kengne, Lucien Honoré Etame Sone, Constant Anatole Pieme. (2022). Surveillance of Resistance to Imipenem and Meropenem in Broad-spectrum Beta-lactamase-producing E. coli Strains Isolated from Urine Samples in Njombe, Cameroon. International Journal of Microbiology and Biotechnology, 7(1), 37-42. https://doi.org/10.11648/j.ijmb.20220701.15

    Copy | Download

    ACS Style

    Marthe Viviane Fonkam; Cédric Gueguim; Michel Kengne; Lucien Honoré Etame Sone; Constant Anatole Pieme. Surveillance of Resistance to Imipenem and Meropenem in Broad-spectrum Beta-lactamase-producing E. coli Strains Isolated from Urine Samples in Njombe, Cameroon. Int. J. Microbiol. Biotechnol. 2022, 7(1), 37-42. doi: 10.11648/j.ijmb.20220701.15

    Copy | Download

    AMA Style

    Marthe Viviane Fonkam, Cédric Gueguim, Michel Kengne, Lucien Honoré Etame Sone, Constant Anatole Pieme. Surveillance of Resistance to Imipenem and Meropenem in Broad-spectrum Beta-lactamase-producing E. coli Strains Isolated from Urine Samples in Njombe, Cameroon. Int J Microbiol Biotechnol. 2022;7(1):37-42. doi: 10.11648/j.ijmb.20220701.15

    Copy | Download

  • @article{10.11648/j.ijmb.20220701.15,
      author = {Marthe Viviane Fonkam and Cédric Gueguim and Michel Kengne and Lucien Honoré Etame Sone and Constant Anatole Pieme},
      title = {Surveillance of Resistance to Imipenem and Meropenem in Broad-spectrum Beta-lactamase-producing E. coli Strains Isolated from Urine Samples in Njombe, Cameroon},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {7},
      number = {1},
      pages = {37-42},
      doi = {10.11648/j.ijmb.20220701.15},
      url = {https://doi.org/10.11648/j.ijmb.20220701.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220701.15},
      abstract = {The emergence and spread of carbapenems resistance Enterobacteriaceae remain a major public health, a real threat as well as a silent tsunami. This phenomenon leading to reduce the therapeutic option and increase the additional cost. The general objective of this study was to determine the frequency of Escherichia coli strains producing broad-spectrum beta lactamases and resistant to carbapenems. We carried out a descriptive, cross sectional and prospective study between August and November 2020 at the Hospital Saint Jean de Malte in Njombe, on a consecutive sample of 249 patients received at the bacteriology unit. Our study population consisted of all patients who came for inpatient or outpatient consultations, were prescribed an cytobacterioligical urine exam, and in whom an E. coli strain was isolated. The identification of E. coli strains was confirmed using the Api 20E mini gallery. Resistance to carbapenems (Meropenem and Imipenem) was defined by determining the Minimal Inhibitory Concentration (MIC) by the microdilution plate method. Of the 249 cytobacterioligical urine exam samples received during our study period, 131 presented a pathogenic germ and E. coli strains were identified in 85 of them. The age of the patients in whom the E. coli strains were identified ranged from 70 to 85 years and the male sex dominated with a frequency of 38.2%. Of these identified E. coli strains, we detected 08 (9.41%) BLSE-producing strains. The resistance rate of the isolated E. coli strains producing BLSE was 75% and 50% for Meropenem and Imipenem respectively. The results of this study underline the urgent need for a regular surveillance system for broad-spectrum antibiotics in our context.},
     year = {2022}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Surveillance of Resistance to Imipenem and Meropenem in Broad-spectrum Beta-lactamase-producing E. coli Strains Isolated from Urine Samples in Njombe, Cameroon
    AU  - Marthe Viviane Fonkam
    AU  - Cédric Gueguim
    AU  - Michel Kengne
    AU  - Lucien Honoré Etame Sone
    AU  - Constant Anatole Pieme
    Y1  - 2022/03/23
    PY  - 2022
    N1  - https://doi.org/10.11648/j.ijmb.20220701.15
    DO  - 10.11648/j.ijmb.20220701.15
    T2  - International Journal of Microbiology and Biotechnology
    JF  - International Journal of Microbiology and Biotechnology
    JO  - International Journal of Microbiology and Biotechnology
    SP  - 37
    EP  - 42
    PB  - Science Publishing Group
    SN  - 2578-9686
    UR  - https://doi.org/10.11648/j.ijmb.20220701.15
    AB  - The emergence and spread of carbapenems resistance Enterobacteriaceae remain a major public health, a real threat as well as a silent tsunami. This phenomenon leading to reduce the therapeutic option and increase the additional cost. The general objective of this study was to determine the frequency of Escherichia coli strains producing broad-spectrum beta lactamases and resistant to carbapenems. We carried out a descriptive, cross sectional and prospective study between August and November 2020 at the Hospital Saint Jean de Malte in Njombe, on a consecutive sample of 249 patients received at the bacteriology unit. Our study population consisted of all patients who came for inpatient or outpatient consultations, were prescribed an cytobacterioligical urine exam, and in whom an E. coli strain was isolated. The identification of E. coli strains was confirmed using the Api 20E mini gallery. Resistance to carbapenems (Meropenem and Imipenem) was defined by determining the Minimal Inhibitory Concentration (MIC) by the microdilution plate method. Of the 249 cytobacterioligical urine exam samples received during our study period, 131 presented a pathogenic germ and E. coli strains were identified in 85 of them. The age of the patients in whom the E. coli strains were identified ranged from 70 to 85 years and the male sex dominated with a frequency of 38.2%. Of these identified E. coli strains, we detected 08 (9.41%) BLSE-producing strains. The resistance rate of the isolated E. coli strains producing BLSE was 75% and 50% for Meropenem and Imipenem respectively. The results of this study underline the urgent need for a regular surveillance system for broad-spectrum antibiotics in our context.
    VL  - 7
    IS  - 1
    ER  - 

    Copy | Download

Author Information
  • School of Health Sciences, Catholic University of Central Africa, Yaoundé, Cameroon

  • Department of Biology, Higher Institute of Medical Technology, Yaoundé, Cameroon

  • School of Health Sciences, Catholic University of Central Africa, Yaoundé, Cameroon

  • Department of Biology, Higher Institute of Medical Technology, Yaoundé, Cameroon

  • Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon

  • Sections