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Genetic Diversity of Circulating Genotypes of PCR Confirmed Neisseria meningitidis Serogroups Amongst Cerebrospinal Meningitis Patients in Parts of Northern Nigeria

Received: 24 February 2022     Accepted: 17 March 2022     Published: 9 April 2022
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Abstract

The genetic potential of an organism is carried in the base sequence of its DNA according to the genetic code. Just as we have defined genotypes in humans, bacterial genotypes are specific and defined too. However, information on circulating N. meningitidis genotypes from northern Nigeria are unavailable in literature. Molecular genotyping methods are used to address two very different kinds of problems associated with disease outbreak (short term or local epidemiology and long term or global epidemiology). The multilocus sequence typing scheme developed for N. meningitidis which is based on DNA sequencing of segments of seven housekeeping loci was used being the gold standard. The PubMLST.org database and Bacterial Isolate Genome Sequence Database software were equally used. Of the 40 genotyped samples, 31 (77.5%) had detectable genotypes; genotype abcZ in three cases (7.5%); adk, four (10%); aroE, three (7.5%); fumC, 16 (40%); pdhC, two (5%); and genotype pdh in three cases (7.5%). The six genotypes encountered were found to be circulating amongst the various serogroups in parts of northern Nigeria which is now publicly reported in literature for the first time in Nigeria. Genotype abcZ was found circulating amongst serogroups A (sacB) and Y (synF); genotype adk amongst serogroups A (sacB), W135 (synG), and Y (synF); genotype aroE amongst serogroups C (synE), W135 (synG), and Y (synF); genotype fumC amongst serogroups A (sacB), B (synD), C (synE), W135 (synG), X (xcbB), and Y (synF); genotype pdhC amongst serogroups B (synD), and X (xcbB); and genotype pgm amongst serogroups B (synD), C (synE), and Y (synF). The utilization of our findings by health authorities would be of great importance for public health strategies such as vaccination. Our knowledge has been widened to acknowledge the presence of bacterial genotypes circulating amongst various N. meningitidis serogroups.

Published in International Journal of Microbiology and Biotechnology (Volume 7, Issue 2)
DOI 10.11648/j.ijmb.20220702.11
Page(s) 51-57
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Genetic Diversity, Bacterial Genotypes, Serogroups, N. meningitidis, CSM, Northern Nigeria

References
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    Iseimokumo Christopher Peletiri, Grace Mebi Ayanbimpe, Eugene Ifeanyichukwu Ikeh. (2022). Genetic Diversity of Circulating Genotypes of PCR Confirmed Neisseria meningitidis Serogroups Amongst Cerebrospinal Meningitis Patients in Parts of Northern Nigeria. International Journal of Microbiology and Biotechnology, 7(2), 51-57. https://doi.org/10.11648/j.ijmb.20220702.11

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    Iseimokumo Christopher Peletiri; Grace Mebi Ayanbimpe; Eugene Ifeanyichukwu Ikeh. Genetic Diversity of Circulating Genotypes of PCR Confirmed Neisseria meningitidis Serogroups Amongst Cerebrospinal Meningitis Patients in Parts of Northern Nigeria. Int. J. Microbiol. Biotechnol. 2022, 7(2), 51-57. doi: 10.11648/j.ijmb.20220702.11

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    AMA Style

    Iseimokumo Christopher Peletiri, Grace Mebi Ayanbimpe, Eugene Ifeanyichukwu Ikeh. Genetic Diversity of Circulating Genotypes of PCR Confirmed Neisseria meningitidis Serogroups Amongst Cerebrospinal Meningitis Patients in Parts of Northern Nigeria. Int J Microbiol Biotechnol. 2022;7(2):51-57. doi: 10.11648/j.ijmb.20220702.11

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  • @article{10.11648/j.ijmb.20220702.11,
      author = {Iseimokumo Christopher Peletiri and Grace Mebi Ayanbimpe and Eugene Ifeanyichukwu Ikeh},
      title = {Genetic Diversity of Circulating Genotypes of PCR Confirmed Neisseria meningitidis Serogroups Amongst Cerebrospinal Meningitis Patients in Parts of Northern Nigeria},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {7},
      number = {2},
      pages = {51-57},
      doi = {10.11648/j.ijmb.20220702.11},
      url = {https://doi.org/10.11648/j.ijmb.20220702.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220702.11},
      abstract = {The genetic potential of an organism is carried in the base sequence of its DNA according to the genetic code. Just as we have defined genotypes in humans, bacterial genotypes are specific and defined too. However, information on circulating N. meningitidis genotypes from northern Nigeria are unavailable in literature. Molecular genotyping methods are used to address two very different kinds of problems associated with disease outbreak (short term or local epidemiology and long term or global epidemiology). The multilocus sequence typing scheme developed for N. meningitidis which is based on DNA sequencing of segments of seven housekeeping loci was used being the gold standard. The PubMLST.org database and Bacterial Isolate Genome Sequence Database software were equally used. Of the 40 genotyped samples, 31 (77.5%) had detectable genotypes; genotype abcZ in three cases (7.5%); adk, four (10%); aroE, three (7.5%); fumC, 16 (40%); pdhC, two (5%); and genotype pdh in three cases (7.5%). The six genotypes encountered were found to be circulating amongst the various serogroups in parts of northern Nigeria which is now publicly reported in literature for the first time in Nigeria. Genotype abcZ was found circulating amongst serogroups A (sacB) and Y (synF); genotype adk amongst serogroups A (sacB), W135 (synG), and Y (synF); genotype aroE amongst serogroups C (synE), W135 (synG), and Y (synF); genotype fumC amongst serogroups A (sacB), B (synD), C (synE), W135 (synG), X (xcbB), and Y (synF); genotype pdhC amongst serogroups B (synD), and X (xcbB); and genotype pgm amongst serogroups B (synD), C (synE), and Y (synF). The utilization of our findings by health authorities would be of great importance for public health strategies such as vaccination. Our knowledge has been widened to acknowledge the presence of bacterial genotypes circulating amongst various N. meningitidis serogroups.},
     year = {2022}
    }
    

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    T1  - Genetic Diversity of Circulating Genotypes of PCR Confirmed Neisseria meningitidis Serogroups Amongst Cerebrospinal Meningitis Patients in Parts of Northern Nigeria
    AU  - Iseimokumo Christopher Peletiri
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    JF  - International Journal of Microbiology and Biotechnology
    JO  - International Journal of Microbiology and Biotechnology
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    AB  - The genetic potential of an organism is carried in the base sequence of its DNA according to the genetic code. Just as we have defined genotypes in humans, bacterial genotypes are specific and defined too. However, information on circulating N. meningitidis genotypes from northern Nigeria are unavailable in literature. Molecular genotyping methods are used to address two very different kinds of problems associated with disease outbreak (short term or local epidemiology and long term or global epidemiology). The multilocus sequence typing scheme developed for N. meningitidis which is based on DNA sequencing of segments of seven housekeeping loci was used being the gold standard. The PubMLST.org database and Bacterial Isolate Genome Sequence Database software were equally used. Of the 40 genotyped samples, 31 (77.5%) had detectable genotypes; genotype abcZ in three cases (7.5%); adk, four (10%); aroE, three (7.5%); fumC, 16 (40%); pdhC, two (5%); and genotype pdh in three cases (7.5%). The six genotypes encountered were found to be circulating amongst the various serogroups in parts of northern Nigeria which is now publicly reported in literature for the first time in Nigeria. Genotype abcZ was found circulating amongst serogroups A (sacB) and Y (synF); genotype adk amongst serogroups A (sacB), W135 (synG), and Y (synF); genotype aroE amongst serogroups C (synE), W135 (synG), and Y (synF); genotype fumC amongst serogroups A (sacB), B (synD), C (synE), W135 (synG), X (xcbB), and Y (synF); genotype pdhC amongst serogroups B (synD), and X (xcbB); and genotype pgm amongst serogroups B (synD), C (synE), and Y (synF). The utilization of our findings by health authorities would be of great importance for public health strategies such as vaccination. Our knowledge has been widened to acknowledge the presence of bacterial genotypes circulating amongst various N. meningitidis serogroups.
    VL  - 7
    IS  - 2
    ER  - 

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Author Information
  • Department of Medical Microbiology, Faculty of Clinical Sciences, College of Health Sciences, University of Jos, Jos, Nigeria

  • Department of Medical Microbiology, Faculty of Clinical Sciences, College of Health Sciences, University of Jos, Jos, Nigeria

  • Department of Medical Microbiology, Faculty of Clinical Sciences, College of Health Sciences, University of Jos, Jos, Nigeria

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